Title | Neuropeptide CGRP Limits Group 2 Innate Lymphoid Cell Responses and Constrains Type 2 Inflammation. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Nagashima, H, Mahlakõiv, T, Shih, H-Y, Davis, FP, Meylan, F, Huang, Y, Harrison, OJ, Yao, C, Mikami, Y, Urban, JF, Caron, KM, Belkaid, Y, Kanno, Y, Artis, D, O'Shea, JJ |
Journal | Immunity |
Date Published | 2019 Jul 16 |
ISSN | 1097-4180 |
Abstract | Innate lymphocytes maintain tissue homeostasis at mucosal barriers, with group 2 innate lymphoid cells (ILC2s) producing type 2 cytokines and controlling helminth infection. While the molecular understanding of ILC2 responses has advanced, the complexity of microenvironmental factors impacting ILC2s is becoming increasingly apparent. Herein, we used single-cell analysis to explore the diversity of gene expression among lung lymphocytes during helminth infection. Following infection, we identified a subset of ILC2s that preferentially expressed Il5-encoding interleukin (IL)-5, together with Calca-encoding calcitonin gene-related peptide (CGRP) and its cognate receptor components. CGRP in concert with IL-33 and neuromedin U (NMU) supported IL-5 but constrained IL-13 expression and ILC2 proliferation. Without CGRP signaling, ILC2 responses and worm expulsion were enhanced. Collectively, these data point to CGRP as a context-dependent negative regulatory factor that shapes innate lymphocyte responses to alarmins and neuropeptides during type 2 innate immune responses. |
DOI | 10.1016/j.immuni.2019.06.009 |
Alternate Journal | Immunity |
PubMed ID | 31353223 |