Drs. Julie Magarian Blander and Steven Lipkin are award recipients of the 2018 Daedalus Fund for Innovation. This award is given to advance early-stage reserach projects that have significant commercial potential for translation of their discoveries into new and more effective treatments for patients. To read more, click here. Donor gift establishes Friedman Center for Nutrition and Inflammation, an innovative cross-campus center dedicated to improving human health through research in the complex relationship between nutrition, inflammation and the development of disease. To read more, click here. The Jill Roberts Institute has been busy with two studies published this month! From the Iliev Lab, the study "Sensing of Fungi by Gut Immune Cells Can Contribute to Airway Allergic Diseases," was published online on November 29 in Cell Host and MicrobeTo read more, click here. From the Longman Lab, the study, "Microbiota-Induced TNF-like Ligand 1A Drives Group 3 Innate Lymphoid Cell-Mediated Barrier Protection and Intestinal T Cell Activation during Colitis," was published on December 11 in Immunity. To read more, click here.  The Kenneth Rainin Foundation awarded Dr. Iliyan Iliev and colleagues from Mount Sinai a $250,000 Synergy Award to examine the composition of the fungal community in babies born to mothers with inflammatory bowel disease. To read more, click here. Dr. Randy Longman received the Irma T. Hirschl Career Scientist Award and the New York Crohn’s Foundation Award.    

The Jill Roberts Institute for Research in Inflammatory Bowel Disease

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Measuring Innate Immune Responses to Bacterial Viability.

TitleMeasuring Innate Immune Responses to Bacterial Viability.
Publication TypeJournal Article
Year of Publication2018
AuthorsMoretti, J, Vabret, N, J Blander, M
JournalMethods Mol Biol
Volume1714
Pagination167-190
Date Published2018
ISSN1940-6029
Abstract

The innate immune system directly senses microbial viability via the detection of a special class of viability-associated pathogen-associated molecular patterns (vita-PAMPs), such as prokaryotic messenger RNA. In the case of Gram-negative bacteria, detection of bacterial viability by phagocytes leads to a unique activation of inflammasome and type I interferon pathways, resulting in a robust pro-inflammatory innate response and a vigorous adaptive immune response. This protocol describes the methods required to study activation of both inflammasome and type I interferon pathways after stimulation of mouse bone marrow-derived macrophages with live or killed Gram-negative and Gram-positive bacteria. It covers the generation and handling of bone marrow-derived macrophages, the culture and killing of bacteria, the preparation of bacterial messenger RNA, and the stimulation of macrophages with live or killed bacteria. Lastly, this protocol describes the techniques employed to measure the hallmarks of inflammasome (secretion of interleukin-1β) and type I interferon (activation of TBK1, IRF3 and secretion of type I interferon) pathways.

DOI10.1007/978-1-4939-7519-8_11
Alternate JournalMethods Mol. Biol.
PubMed ID29177862