The 4th Annual NYC IBD Research Day is September 12, 2018 with an outstanding group of speakers. Registering for the event is free but space is limited. To attend, please visit the event link here. The latest study from the Jill Roberts Institute, "Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses," was published on March 13 in Immunity. To read more, click here.  The latest reviews from the Jill Roberts Institute, "Neuronal–immune system cross-talk in homeostasis" was published on March 30 in Science and "Beyond Host Defense: Emerging Functions of the Immune System in Regulating Complex Tissue Physiology" was published on April 19 in Cell. Dr. Gregory Sonnenberg wins inaugural award from the Society for Mucosal Immunology. To read more, click here.  The Kenneth Rainin Foundation awarded Dr. Iliyan Iliev and colleagues from Mount Sinai a $250,000 Synergy Award to examine the composition of the fungal community in babies born to mothers with inflammatory bowel disease. To read more, click here. Dr. Randy Longman received the Irma T. Hirschl Career Scientist Award and the New York Crohn’s Foundation Award.    

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Measuring Innate Immune Responses to Bacterial Viability.

TitleMeasuring Innate Immune Responses to Bacterial Viability.
Publication TypeJournal Article
Year of Publication2018
AuthorsMoretti, J, Vabret, N, J Blander, M
JournalMethods Mol Biol
Volume1714
Pagination167-190
Date Published2018
ISSN1940-6029
Abstract

The innate immune system directly senses microbial viability via the detection of a special class of viability-associated pathogen-associated molecular patterns (vita-PAMPs), such as prokaryotic messenger RNA. In the case of Gram-negative bacteria, detection of bacterial viability by phagocytes leads to a unique activation of inflammasome and type I interferon pathways, resulting in a robust pro-inflammatory innate response and a vigorous adaptive immune response. This protocol describes the methods required to study activation of both inflammasome and type I interferon pathways after stimulation of mouse bone marrow-derived macrophages with live or killed Gram-negative and Gram-positive bacteria. It covers the generation and handling of bone marrow-derived macrophages, the culture and killing of bacteria, the preparation of bacterial messenger RNA, and the stimulation of macrophages with live or killed bacteria. Lastly, this protocol describes the techniques employed to measure the hallmarks of inflammasome (secretion of interleukin-1β) and type I interferon (activation of TBK1, IRF3 and secretion of type I interferon) pathways.

DOI10.1007/978-1-4939-7519-8_11
Alternate JournalMethods Mol. Biol.
PubMed ID29177862