Submitted by jec3011 on August 9, 2019 - 12:03pm
Title | Genome-based deletion analysis in Aspergillus terreus reveals the acetylaranotin bis-thiomethyltransferase gene. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Sun, W-W, Romsdahl, J, Guo, C-J, Wang, CCC |
Journal | Fungal Genet Biol |
Volume | 119 |
Pagination | 1-6 |
Date Published | 2018 10 |
ISSN | 1096-0937 |
Keywords | Acetyltransferases, Aspergillus, Biosynthetic Pathways, Genome, Fungal, Methyltransferases, Multigene Family, Oxepins, Oxygenases, Piperazines, Sequence Deletion |
Abstract | Acetylaranotin is an epipolythiodiketopiperazine (ETP) secondary metabolite with a broad range of bioactivities. We demonstrated that ATEG_01465.1 located outside of acetylaranotin gene cluster is responsible for catalyzing the S-methylation of its biosynthetic pathway. Combining the previous characterization of acetylaranotin biosynthetic gene cluster together with the identification of its S-methyltransferase provides a means to obtain second-generation acetylaranotin derivatives previously inaccessible. By permutations of targeted deletions of ATEG_01465.1, acetyltransferase (AtaH), and benzoate hydroxylase (AtaY), three novel acetylaranotin derivatives were produced by Aspergillus terreus. |
DOI | 10.1016/j.fgb.2018.08.001 |
Alternate Journal | Fungal Genet. Biol. |
PubMed ID | 30096370 |