Title | CCR7-dependent trafficking of RORγ⁺ ILCs creates a unique microenvironment within mucosal draining lymph nodes. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Mackley, EC, Houston, S, Marriott, CL, Halford, EE, Lucas, B, Cerovic, V, Filbey, KJ, Maizels, RM, Hepworth, MR, Sonnenberg, GF, Milling, S, Withers, DR |
Journal | Nat Commun |
Volume | 6 |
Pagination | 5862 |
Date Published | 2015 Jan 09 |
ISSN | 2041-1723 |
Keywords | Animals, CD4-Positive T-Lymphocytes, Cell Movement, Female, Immunity, Innate, Intestines, Light, Lymph Nodes, Lymphocytes, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Fluorescence, Mucous Membrane, Nuclear Receptor Subfamily 1, Group F, Member 3, Receptors, CCR7 |
Abstract | Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms. |
DOI | 10.1038/ncomms6862 |
Alternate Journal | Nat Commun |
PubMed ID | 25575242 |
PubMed Central ID | PMC4354100 |
Grant List | 106122 / / Wellcome Trust / United Kingdom DP5OD012116 / OD / NIH HHS / United States DP5 OD012116 / OD / NIH HHS / United States MR/K021095/1 / / Medical Research Council / United Kingdom 095831 / / Wellcome Trust / United Kingdom G9818340 / / Medical Research Council / United Kingdom / / Wellcome Trust / United Kingdom |