The latest study from the Jill Roberts Institute, "The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation," was published on September 6 in Nature. To read more, click here.     Dr. Gregory Sonnenberg wins inaugural award from the Society for Mucosal Immunology. To read more, click here.  The Kenneth Rainin Foundation awarded Dr. Iliyan Iliev and colleagues from Mount Sinai a $250,000 Synergy Award to examine the composition of the fungal community in babies born to mothers with inflammatory bowel disease. To read more, click here. Dr. Randy Longman received the Irma T. Hirschl Career Scientist Award and the New York Crohn’s Foundation Award.    

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A pool of central memory-like CD4 T cells contains effector memory precursors.

TitleA pool of central memory-like CD4 T cells contains effector memory precursors.
Publication TypeJournal Article
Year of Publication2003
AuthorsJ Blander, M, Sant'Angelo, DB, Metz, D, Kim, S-W, Flavell, RA, Bottomly, K, Janeway, CA
JournalJ Immunol
Volume170
Issue6
Pagination2940-8
Date Published2003 Mar 15
ISSN0022-1767
KeywordsAmino Acid Substitution, Animals, Antigens, CD44, Antigens, CD45, CD4-Positive T-Lymphocytes, Cell Division, Cells, Cultured, Conalbumin, Cytokines, Dose-Response Relationship, Immunologic, Genes, T-Cell Receptor alpha, Immunization, Secondary, Immunologic Memory, Immunophenotyping, Interphase, L-Selectin, Leucine, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Mice, Transgenic, Peptide Fragments, Receptors, CCR7, Receptors, Chemokine, Receptors, Interleukin-2, Serine, Stem Cells, T-Lymphocyte Subsets
Abstract

The L51S mutation in the D10.G4.1 TCR alpha-chain reduces the affinity of the TCR to its ligand by affecting the interactions among the TCR, the beta-chain of I-A(k), and the bound peptide. We show that this mutation drives the generation of a pool of memory CD44(high)CD62L(neg)CD45RB(neg) CD4 TCR transgenic T cells. Their activation threshold is low, such that they proliferate in response to lower concentrations of agonist peptides than naive L51S CD4 T cells. Unlike effector memory CD4 T cells, however, they lack immediate effector function in response to TCR stimulation. These cells express IL-2R alpha only after culture with specific peptide. Although they can be recovered from lymph nodes, the majority lack the expression of the lymph node homing receptor CCR7. When these cells receive a second TCR stimulation in vitro, they differentiate into potent Th2-like effector cells, producing high levels of IL-4 at doses of agonist peptide too low to stimulate cytokine release from similarly differentiated naive L51S CD4 T cells. Having these properties, the L51S TCR transgenic memory CD4 T cells cannot be classified as either strict central memory or effector memory, but, rather, as a pool of memory T cells containing effector memory precursors.

Alternate JournalJ. Immunol.
PubMed ID12626545